How to Reconstitute TB-500 | 5mg & 10mg Vial Guide

How to Reconstitute TB-500: Research Guide (5mg & 10mg Vials)

Introduction

TB-500 (Thymosin Beta-4 fragment) is a peptide widely studied for its effects on muscle repair, angiogenesis, and systemic regeneration. Like many peptides, TB-500 is supplied as a lyophilized (freeze-dried) powder, requiring reconstitution before use in laboratory research.

This guide explains the general process of reconstituting TB-500, with examples for 5 mg and 10 mg vials.

⚠️ Important: TB-500 is for laboratory research use only. It is not approved for human use.

What Does Reconstitution Mean?

  • Converting lyophilized TB-500 powder into liquid form using a sterile diluent (commonly bacteriostatic water in research settings).

  • Ensures precise concentration for dosing accuracy in experiments.

Example: Reconstituting a 5 mg TB-500 Vial

  1. Select Diluent Volume

    • Researchers often use 1 ml, 2 ml, or 5 ml of bacteriostatic water.

  2. Calculate Concentration

    • 5 mg TB-500 + 1 ml water = 5 mg/ml.

    • 5 mg TB-500 + 2 ml water = 2.5 mg/ml.

    • 5 mg TB-500 + 5 ml water = 1 mg/ml.

  3. Add Diluent Slowly

    • Inject sterile water down the side of the vial to prevent foaming.

  4. Swirl Gently

    • Do not shake — swirl until dissolved.

Example: Reconstituting a 10 mg TB-500 Vial

  1. Select Diluent Volume

    • Common options: 2 ml, 4 ml, or 10 ml of bacteriostatic water.

  2. Calculate Concentration

    • 10 mg TB-500 + 2 ml water = 5 mg/ml.

    • 10 mg TB-500 + 4 ml water = 2.5 mg/ml.

    • 10 mg TB-500 + 10 ml water = 1 mg/ml.

  3. Swirl Gently

    • Avoid shaking to maintain peptide stability.

Quick Reference: Reconstitution Chart

Vial Size Diluent Volume Final Concentration
5 mg 1 ml 5 mg/ml
5 mg 2 ml 2.5 mg/ml
5 mg 5 ml 1 mg/ml
10 mg 2 ml 5 mg/ml
10 mg 4 ml 2.5 mg/ml
10 mg 10 ml 1 mg/ml

FAQs

Can TB-500 be shaken during mixing?
No — swirl gently to protect peptide structure.

What’s the best diluent?
Most studies use bacteriostatic water, but research protocols may vary.

How should reconstituted TB-500 be stored?
Generally refrigerated at 2–8°C. Stability depends on study duration.

Is TB-500 legal for clinical use?
No. It is available strictly for laboratory research.

References & Further Reading

  • Goldstein, A. L. et al. (2012). Thymosin Beta-4 research and regenerative biology. Ann N Y Acad Sci.

  • PubMed: TB-500 studies.

Final Thoughts

Reconstituting TB-500 is simple: add diluent, calculate concentration, and swirl. Whether working with 5 mg or 10 mg vials, correct preparation ensures accuracy in laboratory studies.

🔬 Looking for premium TB-500 for your lab?
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